| Registro completo |
| Provedor de dados: |
J. Venom. Anim. Toxins
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| País: |
Brazil
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| Título: |
Rabbit antivenom efficacy against myotoxic and neurotoxic activities of Bothrops jararacussu venom and bothropstoxin-I
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| Autores: |
Oshima-Franco,Y.
Leite,G. B.
Valério,A. A.
Hyslop,S.
Andriao-Escarso,S.
Giglio,J. R.
Prado-Franceschi,J.
Cruz-Höfling,M. A.
Rodrigues-Simioni,L.
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| Data: |
2002-01-01
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| Ano: |
2002
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| Palavras-chave: |
Bothrops venom
Myotoxin
Neutralization
Neuromuscular junction
Histological analysis
Bothrops jararacussu
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| Resumo: |
Bothrops jararacussu venom and its major toxin bothropstoxin-I (BthTX-I) possess myotoxic and neurotoxic properties. The efficacy of a rabbit antivenom raised against B. jararacussu venom in the neutralization of physiological, biochemical, and morphological changes induced by the venom and its major toxin BthTX-I was studied in mouse isolated phrenic nerve-diaphragm (PND) and extensor digitorum longus (EDL) preparations. The times required for 50% neuromuscular blockade in PND and EDL preparations for venom were 70+11.5 (S.E.M., n=5) min and 58+8 (n=16) (50 mu g/mL), and for BthTX-I 31+6 (n=3) min and 30+3 (n=5) min (20 mu g/mL), respectively. After 120 min incubation, creatine kinase (CK) concentrations in solution containing the EDL preparations were 3464+346 U/L after exposure to venom (50 mu g/mL, n=5) and 3422+135 U/L to BthTX-I (20mu g/mL, n=4), respectively. Rabbit antivenom dose-dependently neutralized venom and toxin-induced neuromuscular blockade in both preparations and effectively prevented venom and toxin-induced CK release from EDL. Histological analysis showed that rabbit antivenom neutralized morphological damage caused by B. jararacussu venom and BthTX-I in EDL preparations. These results indicate that rabbit antivenom effectively neutralized the biological activities of B. jararacussu venom and BthTX-I.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000200004
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| Editor: |
Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP
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| Relação: |
10.1590/S0104-79302002000200004
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| Formato: |
text/html
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| Fonte: |
Journal of Venomous Animals and Toxins v.8 n.2 2002
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| Direitos: |
info:eu-repo/semantics/openAccess
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